CF800573

antibody from Invitrogen Antibodies

Targeting: MMADHC C2orf25, cblD, CL25022

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

CF800573

Provider

Invitrogen Antibodies

Product name

MMADHC Monoclonal Antibody (OTI1G4), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1G4

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMADHC (RC204801, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MMADH. Positive lysates LY414395 (100 µg) and LC414395 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-MMADHC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).