Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF2326 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TSG-6 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse TSG-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human TSG-6 is observed and less than 2% cross-reactivity with recombinant mouse TSG-14 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O08859
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mesenchymal Stromal Cells Inhibit Neutrophil Effector Functions in a Murine Model of Ocular Inflammation.
Mittal SK, Mashaghi A, Amouzegar A, Li M, Foulsham W, Sahu SK, Chauhan SK
Investigative ophthalmology & visual science 2018 Mar 1;59(3):1191-1198
Investigative ophthalmology & visual science 2018 Mar 1;59(3):1191-1198
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TSG-6 in Mouse Splenocytes. TSG-6 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse TSG-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2326) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.