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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PAB10275 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10275, RRID:AB_1712996
- Product name
- Rif1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Rif1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification and characterisation of mRif1: a mouse telomere-associated protein highly expressed in germ cells and embryo-derived pluripotent stem cells.
Adams IR, McLaren A
Developmental dynamics : an official publication of the American Association of Anatomists 2004 Apr;229(4):733-44
Developmental dynamics : an official publication of the American Association of Anatomists 2004 Apr;229(4):733-44
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using Rif1 polyclonal antibody (Cat # PAB10275) shows detection of a band ~ 265 KDa corresponding to mouse Rif1 (arrowhead).Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown).Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
