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Learn210-401-C22
antibody from Rockland Immunochemicals, Inc.
Targeting: TNFSF11
CD254, ODF, OPGL, RANKL, TRANCE
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 210-401-C22 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#210-401-C22, RRID:AB_10704514
- Product name
- Anti-Mouse RANK-L (RABBIT) Antibody - 210-401-C22
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Anti-mouse RANKL antibody in western blot shows detection of recombinant mouse RANKL raised in E.coli. Recombinant truncated protein (0.1 µg, 19.9 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Rockland's Anti-Mouse RANKL antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
- Validation comment
- Western Blot
