Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 662302 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#662302, RRID:AB_2564246
- Product name
- Purified anti-DDX58 (RIG-I)
- Antibody type
- Monoclonal
- Antigen
- Partial DDX58 recombinant protein (1-228 a.a.) expressed in E. coli.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 9F1B20
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from THP-1 cells untreated (lane 1) or treated (lane 2) with 20 ng/mL PMA for 72 hours and 1 µg/mL LPS for 24 hours were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-DDX58 (clone 9F1B20) antibody. Proteins were visualized using an anti-mouse-IgG secondary (cat#405306) conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated