AP32907PU-N

antibody from Acris Antibodies GmbH

Targeting: DDX58 DKFZp434J1111, FLJ13599, RIG-1, RIG-I, RIG1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP32907PU-N

Provider

Acris Antibodies GmbH

Product name

anti DDX58 (200-250)

Antibody type

Polyclonal

Antigen

A portion of amino acid 200-250 of Human RIG-I

Reactivity

Human, Simian

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

References

Submitted references

Cytokine profiles, signalling pathways and effects of fluticasone propionate in respiratory syncytial virus-infected human foetal lung fibroblasts.

Seki E, Yoshizumi M, Tanaka R, Ryo A, Ishioka T, Tsukagoshi H, Kozawa K, Okayama Y, Okabe-Kado J, Goya T, Kimura H
Cell biology international 2013 Apr;37(4):326-39

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of 293 lysate in the 1) absence and 2) presence of immunizing peptide using RIG-I antibody at 3 µg/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human breast stained with RIG-I antibody at 10 µg/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human uterus stained with RIG-I antibody at 10 µg/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.