Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 12596-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OLFM3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: NLSAVLTGI QEEIGAYDYE ELHQRVLSLE TRLRDCMKKL TCGKLMKITG PVTVKTSGTR FGAWMTDPLA SEKNNRVWYM DSYTNNKIVR EYKSIADFVS GAESRTYNLP FKWAGTNHVV YNGSLYFNKY QSNIIIKYSF DMGRVLAQRS LEYAGFHNVY PYTWGGFSDI DLMADEIGLW AVYATNQNAG NIVISQLNQD TLEVMKSWST GYPKRSAGES FMICGTLYVT NSHLTGAKVY YSYSTKTSTY EYTDIPFHNQ YFHISMLDYN ARDRALCAWN NGHQVLFNVT LFHIIKTEDD T (179-478 aa encoded by BC022531)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP ( OLFM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A2780 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using OLFM3 antibody 12596-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12596-1-AP ( OLFM3 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12596-1-AP ( OLFM3 Antibody) at dilution of 1:50 (under 40x lens).