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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 66605-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERMN Monoclonal Antibody (2B1C1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- The predicted MW of ERMN is 33 kDa, while western blot analyses of different tissue lysates using 66605-1-Ig antibody to ERMN detected a 50 kDa single band protein as a result of phosphorylation modification.
- Antibody clone number
- 2B1C1
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various tissues were subjected to SDS PAGE followed by western blot with 66605-1-IG (ERMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various tissues were subjected to SDS PAGE followed by western blot with 66605-1-IG (ERMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66605-1-IG (ERMN antibody) at dilution of 1:300 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
