BS9011

antibody from Bioworld Technology, Inc

Targeting: EIF5A2

Western blot (Data presented on provider website)

Antibody Data

Product number

BS9011

Provider

Bioworld Technology, Inc

Product name

eIF5A2 (Y127) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide, corresponding to amino acids 96-140 of Human eIF5A2.

Description

In mammalian cells, translation is controlled at the level of polypeptide chain initiation by eukaryotic initiation factors. The translation initiation factor 5 (eIF5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal subunit, a function necessary for the subsequent joining of the 40S and 60S subunits to form the 80S initiation complex. eIF4E specifically binds to the mRNA cap to promote unwinding and exposure of the AUG-initiation codon. Overexpression of eIF4E can lead to cell transformation and tumorigenesis. An additional initiation factor, eIF2, is present as a heterotrimer composed of eIF2Ï«, eIF2ÉÇ and eIF2ÎÛ subunits. This heterotrimer forms a complex with GTP and tRNA which then binds to the 40S ribosomal subunit. After the formation of the 80S initiation complex, eIF2 is hydrolyzed and eIF2-GDP is released from the complex. eIF2-GDP is subsequently converted to eIF2-GTP, a reaction catalyzed by eIF2B, and is then available to catalyze another round of initiation.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.