H00056648-M01

antibody from Abnova Corporation

Targeting: EIF5A2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00056648-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00056648-M01, RRID:AB_509170

Product name

EIF5A2 monoclonal antibody (M01), clone 1E7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant EIF5A2.

Antigen sequence

GKKYEDICPSTHNMDVPNIKRNDYQLICIQDGYLS
LLTETGEVREDLKLPEGELGKEIEGKYNAGEDVQV
SVMCAMSEEYAVAIKPCK


Isotype

IgG

Antibody clone number

1E7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

A hypusine-eIF5A-PEAK1 switch regulates the pathogenesis of pancreatic cancer.

Fujimura K, Wright T, Strnadel J, Kaushal S, Metildi C, Lowy AM, Bouvet M, Kelber JA, Klemke RL
Cancer research 2014 Nov 15;74(22):6671-81

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of EIF5A2 expression in transfected 293T cell line by EIF5A2 monoclonal antibody (M01), clone 1E7.Lane 1: EIF5A2 transfected lysate(16.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged EIF5A2 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol