Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-117691 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MFN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.39 mg/mL
- Storage
- -20°C or -80°C if preferred
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MFN1 using MFN1 Polyclonal Antibody (Product # PA5-117691) at 1.1 µg/mL. Lane 1: Human placenta tissue. Lane 2: Jurkat whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: K562 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:10,000 dilution. Predicted band size: 85, 42, 72 kDa. Observed band size: 85 kDa
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MFN1 using MFN1 Polyclonal Antibody (Product # PA5-117691) at 1.1 µg/mL. Lane 1: Human placenta tissue. Lane 2: Jurkat whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: K562 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:10,000 dilution. Predicted band size: 85, 42, 72 kDa. Observed band size: 85 kDa
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immnohistochemistry (Paraffin) analysis of MFN1 in paraffin-embedded human testis tissue using MFN1 Polyclonal Antibody (Product # PA5-117691) diluted at 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitating MFN1 in Jurkat whole cell lysate. Lane 1: Rabbit control IgG instead of (1 µg) Jurkat whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1:50,000). Lane 2: MFN1 Polyclonal Antibody (Product # PA5-117691) (4 µg) + Jurkat whole cell lysate (500 µg). Lane 3: Jurkat whole cell lysate (20 µg).