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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- GTX102474 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102474, RRID:AB_11165724
- Product name
- TMS1 antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE GTX102474 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TMS1 antibody detects TMS1 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg whole cell lysate/extract of 3xFlag-human TMS1-transfected 293T cells12 % SDS-PAGETMS1 antibody (GTX102474) dilution: 1:20000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with TMS1 antibody [N1C3] (GTX102474) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TMS1 antibody [N1C3] detects TMS1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: TMS1 protein stained by TMS1 antibody [N1C3] (GTX102474) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
