Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 653903 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#653903, RRID:AB_2564507
- Product name
- PE anti-ASC (TMS-1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant human ASC (aa 113-195).
- Description
- Apoptosis-associated speck-like protein containing a CARD (ASC), also known as target of methylation-mediated silencing (TMS1) and PYD and CARD domain containing protein (PYCARD), is a 22 kD pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD). The TMS1 gene was originally found to be aberrantly methylated and silenced in various cancer cells. Expression of ASC can be induced by pro-apoptotic/inflammatory stimuli. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. During apoptosis, ASC translocated from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis. The adaptor molecule ASC mediates AIM2-dependent caspase-1 activation. Recent studies also show that AIM2/ASC complex acts as a novel caspase-8 activation platform. Moreover, emerging reports show novel roles for ASC in disease conditions such as arthritis.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- HASC-71
- Vial size
- 25 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human acute promyelocytic leukemia cell line, HL-60, was treated with Fixation Buffer and Permeabilization Wash Buffer, and then was stained with anti-human ASC (clone HASC-71) PE (filled histogram) or mouse IgG1, k PE isotype control (open histogram).
- Conjugate
- Yellow dye