H00025801-M01

antibody from Abnova Corporation

Targeting: GCA GCL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00025801-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00025801-M01, RRID:AB_606283

Product name

GCA monoclonal antibody (M01), clone 2F5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant GCA.

Antigen sequence

MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILL
DGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDA
EELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDH
TGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVE
HHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFF
DDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDF
LQGTMAI


Isotype

IgG

Antibody clone number

2F5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GCA monoclonal antibody (M01), clone 2F5 Western Blot analysis of GCA expression in MCF-7 ( Cat # L046V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to GCA on MCF-7 cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol