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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00025801-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00025801-M01, RRID:AB_2107186
- Product name
- Mouse Monoclonal Grancalcin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GCA (2F5)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Functional profiling reveals critical role for miRNA in differentiation of human mesenchymal stem cells.
Schoolmeesters A, Eklund T, Leake D, Vermeulen A, Smith Q, Force Aldred S, Fedorov Y
PloS one 2009;4(5):e5605
PloS one 2009;4(5):e5605
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Grancalcin Antibody (2F5) [H00025801-M01] - GCA monoclonal antibody (M01), clone 2F5 Analysis of GCA expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Grancalcin Antibody (2F5) [H00025801-M01] - Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Grancalcin Antibody (2F5) [H00025801-M01] - Analysis of monoclonal antibody to GCA on MCF-7 cell . Antibody concentration 10 ug/ml.
