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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB110-11473 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-11473, RRID:AB_2119086
- Product name
- Rabbit Polyclonal HSP10/EPF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified. Rabbit Polyclonal to Cpn10
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Porcine, Rabbit, Sheep, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HSP10/EPF Antibody [NB110-11473] - analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: HSP10/EPF Antibody [NB110-11473] - analysis using Rabbit Anti-Cpn10 Polyclonal Antibody . Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..
