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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-29566 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LAMP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, Molt-4. Predicted reactivity: Rhesus Monkey (94%), Chimpanzee (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Hydroxypropyl-beta and -gamma cyclodextrins rescue cholesterol accumulation in Niemann-Pick C1 mutant cell via lysosome-associated membrane protein 1.
Singhal A, Szente L, Hildreth JEK, Song B
Cell death & disease 2018 Oct 3;9(10):1019
Cell death & disease 2018 Oct 3;9(10):1019
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LAMP3 Polyclonal Antibody (Product # PA5-29566). Sample (30 µg of whole cell lysate). Lane A: HeLa. 10% SDS PAGE. LAMP3 Polyclonal Antibody (Product # PA5-29566) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of LAMP-3/DC-LAMP in methanol-fixed HeLa cells using a LAMP-3/DC-LAMP polyclonal antibody (Product # PA5-29566) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 HPbetaCD or HPgammaCD treatment induces LAMP-1 expression and causes a change in lysosomal positioning in NPC1 mutant cells. The healthy (wild-type) cells or NPC1 -/- cells treated with HPbetaCD or HPgammaCD for 72 h were lysed for immunoblotting ( a , b ) or stained for LAMP-1 ( c ). a The representative western blot and bar diagram of three experiments show that wild-type or NPC1 -/- cells treated with either HPbetaCD or HPgammaCD showed significantly higher levels of LAMP-1 expression ( p < 0.001). The error bars shows mean +- S.E.M. of fold change calculated by densitometry analysis. b The representative western blot of three experiments shows that neither HPbetaCD nor HPgammaCD treatment changed expression of LAMP-2 or LAMP-3 in wild-type or NPC1 -/- cells. c Immunostaining micrographs show LAMP-1 (red, a lysosome marker) and DAPI (blue, a nucleus marker) staining. The arrow represents the distribution of LAMP-1 from the center of the nuclei. Data depict that the LAMP-1 protein is mostly confined to the area near the nuclear envelop in control NPC1 -/- cells, whereas it is distributed more widely throughout the cytoplasm when cells were treated with either HPbetaCD or HPgammaCD. Images are a representative of at least three random fields of three experimental replicates. Scale bar = 100 mum. d The LAMP-1 distribution per cell was quantified by measuring the area of fluorescence. Data are mean +- S.E.M. and representative of three experiments
