Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00010768-M05 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010768-M05, RRID:AB_536656
- Product name
- Mouse Monoclonal AHCYL1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. AHCYL1 - S-adenosylhomocysteine hydrolase-like 1
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Restoration of Na+/H+ exchanger NHE3-containing macrocomplexes ameliorates diabetes-associated fluid loss.
Protein extraction of formalin-fixed, paraffin-embedded tissue enables robust proteomic profiles by mass spectrometry.
He P, Zhao L, Zhu L, Weinman EJ, De Giorgio R, Koval M, Srinivasan S, Yun CC
The Journal of clinical investigation 2015 Sep;125(9):3519-31
The Journal of clinical investigation 2015 Sep;125(9):3519-31
Protein extraction of formalin-fixed, paraffin-embedded tissue enables robust proteomic profiles by mass spectrometry.
Scicchitano MS, Dalmas DA, Boyce RW, Thomas HC, Frazier KS
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Sep;57(9):849-60
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Sep;57(9):849-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AHCYL1 Antibody (5D6) [H00010768-M05] - Analysis of AHCYL1 expression in transfected 293T cell line by AHCYL1 monoclonal antibody (M05), clone 5D6. Lane 1: AHCYL1 transfected lysatE (69 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AHCYL1 Antibody (5D6) [H00010768-M05] - Analysis of AHCYL1 expression in HeLa (Cat # L013V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: AHCYL1 Antibody (5D6) [H00010768-M05] - Detection limit for recombinant GST tagged AHCYL1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AHCYL1 Antibody (5D6) [H00010768-M05] - Analysis of monoclonal antibody to AHCYL1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml