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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4827 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4827, RRID:AB_1580004
- Product name
- ST13 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ST13.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The Hsp organizer protein hop enhances the rate of but is not essential for glucocorticoid receptor folding by the multiprotein Hsp90-based chaperone system.
The molecular chaperones Hsp90 and Hsc70 are both necessary and sufficient to activate hormone binding by glucocorticoid receptor.
Hop as an adaptor in the heat shock protein 70 (Hsp70) and hsp90 chaperone machinery.
Morishima Y, Kanelakis KC, Silverstein AM, Dittmar KD, Estrada L, Pratt WB
The Journal of biological chemistry 2000 Mar 10;275(10):6894-900
The Journal of biological chemistry 2000 Mar 10;275(10):6894-900
The molecular chaperones Hsp90 and Hsc70 are both necessary and sufficient to activate hormone binding by glucocorticoid receptor.
Rajapandi T, Greene LE, Eisenberg E
The Journal of biological chemistry 2000 Jul 21;275(29):22597-604
The Journal of biological chemistry 2000 Jul 21;275(29):22597-604
Hop as an adaptor in the heat shock protein 70 (Hsp70) and hsp90 chaperone machinery.
Chen S, Smith DF
The Journal of biological chemistry 1998 Dec 25;273(52):35194-200
The Journal of biological chemistry 1998 Dec 25;273(52):35194-200
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ST13 polyclonal antibody (Cat # PAB4827) pre-incubated with (Lane 2) and without (Lane 1) blocking peptide in A2058 cell lysate. ST13 (arrow) was detected using the purified polyclonal antibody (1 : 240 dilution) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with ST13 polyclonal antibody (Cat # PAB4827) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
