Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27723 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DVL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116. Predicted reactivity: Mouse (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.99 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DVL3 Polyclonal Antibody (Product # PA5-27723). Sample (30 µg of whole cell lysate). Lane A: HCT116 7.5% SDS PAGE. DVL3 Polyclonal Antibody (Product # PA5-27723) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Dishevelled 3 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Dishevelled 3 polyclonal antibody (Product # PA5-27723) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of DVL3 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: DVL3 Polyclonal Antibody (Product # PA5-27723) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DVL3 Polyclonal Antibody detects Dishevelled 3 protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. DVL3 Polyclonal Antibody (Product # PA5-27723) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.