Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN951920 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Dishevelled, Dsh Homolog 3 (Drosophila) (DVL3) (C-Term), (AA 537-565) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 537-565 amino acids from the C-terminal region of Human DVL3.
- Description
- Affinity Chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 537-565
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Activation of the non-canonical Dvl-Rac1-JNK pathway by Frizzled homologue 10 in human synovial sarcoma.
Differential mediation of the Wnt canonical pathway by mammalian Dishevelleds-1, -2, and -3.
Fukukawa C, Nagayama S, Tsunoda T, Toguchida J, Nakamura Y, Katagiri T
Oncogene 2009 Feb 26;28(8):1110-20
Oncogene 2009 Feb 26;28(8):1110-20
Differential mediation of the Wnt canonical pathway by mammalian Dishevelleds-1, -2, and -3.
Lee YN, Gao Y, Wang HY
Cellular signalling 2008 Feb;20(2):443-52
Cellular signalling 2008 Feb;20(2):443-52
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of Dishevelled-3 / DVL3 Antibody (C-term) Cat.-No AP51346PU-N in K562 cell line lysates (35ug/lane). This demonstrates the DVL3 antibody detected the DVL3 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Dishevelled-3 / DVL3 Antibody (C-term) Cat.-No AP51346PU-N with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human breast carcinoma reacted with Dishevelled-3 / DVL3 Antibody (C-term) Cat.-No AP51346PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.