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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA503015 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503015, RRID:AB_11127990
- Product name
- AP2M1 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)
- Antibody type
- Monoclonal
- Description
- AP2M1 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AP2M1
- Isotype
- IgG
- Antibody clone number
- OTI1F10
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (RC201377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-AP2M1 monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-AP2M1 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-AP2M1 mouse monoclonal antibody (TA503015) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AP2M1(RC201377).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201377 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AP2M1 antibody(TA503015), and then analyzed by flow cytometry.
- Validation comment
- FC
