H00010170-M06

antibody from Abnova Corporation

Targeting: DHRS9 3alpha-HSD, RDH15, RDHL, RETSDR8, SDR9C4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010170-M06

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010170-M06, RRID:AB_1112352

Product name

DHRS9 monoclonal antibody (M06), clone 3E3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant DHRS9.

Antigen sequence

MLFWVLGLLILCGFLWTRKGKLKIEDITDKYIFIT
GCDSGFGNLAARTFDKKGFHVIAACLTESGSTALK
AETSERLRTVLLDVTDPENVKRTAQWVKNQVGEKG
LWGLINNAGVPGVLAPTDWLTLEDYREPIEVNLFG
LISVTLNMLPLVKKAQGRVINVSSVGGRLAIVGGG
YTPSKYAVEGFNDSLRRDMKAFGVHVSCIEPGLFK
TNLADPVKVIEKKLAIWEQLSPDIKQQYGEGYIEK
SLDKLKGNKSYVNMDLSPVVECMDHALTSLFPKTH
YAAGKDAKIFWIPLSHMPAALQDFLLLKQKAELAN
PKAV


Isotype

IgG

Antibody clone number

3E3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DHRS9 expression in transfected 293T cell line by DHRS9 monoclonal antibody (M06), clone 3E3.Lane 1: DHRS9 transfected lysate(35.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged DHRS9 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of DHRS9 transfected lysate using anti-DHRS9 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DHRS9 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol