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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-50259 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-50259, RRID:AB_10012459
- Product name
- Rabbit Polyclonal CaMKIIN2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~50k a-CaM Kinase II and the ~60k R-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the X-phosphatase treatment
- Reactivity
- Human, Mouse, Rat, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CaMKIIN2 [p Thr286] Antibody [NBP1-50259] - Analysis of rat brain lysate, showing specific immunolabeling of the ~50k a- and the ~60k b-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with l-Ptase.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CaMKIIN2 [p Thr286] Antibody [NBP1-50259] - Analysis of anti-CAMK2 antibody with human spleen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CaMKIIN2 [p Thr286] Antibody [NBP1-50259] - Analysis of anti-CAMK2 antibody with human brain, cortex at dilution 1:200.
