Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 14748-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GLGLNHLGK GEAIEAILAA LEVVSEPFRS FANTLVDVCA YAGSGNVLKV QQLLHICSEH FDSKEKEEDK DKKEKKDKDK KEAPADMGAH QGVAVLGIAL IAMGEEIGAE MALRTFGHLL RYGEPTLRRA VPLALALISV SNPRLNILDT LSKFSHDADP EVSYNSIFAM GMVGSGTNNA RLAAMLRQLA QYHAKDPNNL FMVRLAQGLT HLGKGTLTLC PYHSDRQLMS QVAVAGLLTV LVSFLDVRNI ILGKSHYVLY GLVAAMQPRM LVTFDEELRP LPVSVRVGQA VDVVGQAGKP KTITGFQTHT TPVLLAHGER AELATEEFLP VTPILEGFVI LRKNPNYDL (561-908 aa encoded by BC002368)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HL-60 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP ( PSMD2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 14748-1-AP (PSMD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 14748-1-AP) (PSMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PSMD2 (IP:14748-1-AP, 4ug; Detection:14748-1-AP 1:1000) with K-562 cells lysate 3320ug.