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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [2]
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- Product number
- PA5-93307 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR2H Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MAGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI ASTLYEDGTL DDGEYNPTDD RPSRADQFEY VMYGKVYRIE GDETSTEAAT RLSAYVSYGG LLMRLQGDAN NLHGFEVDSR VYLLMKKLAF; Positive Samples: SW480, MCF-7, HL-60, 293T, Mouse spleen, Mouse brain, Mouse thymus; Cellular Location: Nucleus, nucleolus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.33 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using POLR2H Polyclonal antibody (Product # PA5-93307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of POLR2H in extracts of various cell lines using POLR2H Polyclonal Antibody (Product # PA5-93307) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of POLR2H was performed in 200 µg extracts of MCF7 cells using POLR2H Polyclonal Antibody (Product # PA5-93307). Western blot was performed from the immunoprecipitate using POLR2H Polyclonal Antibody at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of POLR2H was performed in 200 µg extracts of MCF7 cells using POLR2H Polyclonal Antibody (Product # PA5-93307). Western blot was performed from the immunoprecipitate using POLR2H Polyclonal Antibody at a dilution of 1:1000.
