Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF758 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Chordin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Chordin in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Oct4 is required ~E7.5 for proliferation in the primitive streak.
Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos.
Chordin-induced lineage plasticity of adult SVZ neuroblasts after demyelination.
Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field.
DeVeale B, Brokhman I, Mohseni P, Babak T, Yoon C, Lin A, Onishi K, Tomilin A, Pevny L, Zandstra PW, Nagy A, van der Kooy D
PLoS genetics 2013 Nov;9(11):e1003957
PLoS genetics 2013 Nov;9(11):e1003957
Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos.
Zakin L, Chang EY, Plouhinec JL, De Robertis EM
Developmental biology 2010 Nov 1;347(1):204-15
Developmental biology 2010 Nov 1;347(1):204-15
Chordin-induced lineage plasticity of adult SVZ neuroblasts after demyelination.
Jablonska B, Aguirre A, Raymond M, Szabo G, Kitabatake Y, Sailor KA, Ming GL, Song H, Gallo V
Nature neuroscience 2010 May;13(5):541-550
Nature neuroscience 2010 May;13(5):541-550
Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field.
Reversade B, De Robertis EM
Cell 2005 Dec 16;123(6):1147-60
Cell 2005 Dec 16;123(6):1147-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chordin in Mouse Liver. Chordin was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Goat Anti-Mouse Chordin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF758) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.