10598-1-AP

antibody from Invitrogen Antibodies

Targeting: GORASP2 GOLPH6, GRASP55, GRS2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10598-1-AP

Provider

Invitrogen Antibodies

Product name

GORASP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LIETHEAKP LKLYVYNTDT DNCREVIITP NSAWGGEGSL GCGIGYGYLH RIPTRPFEEG KKISLPGQMA GTPITPLKDG FTEVQLSSVN PPSLSPPGTT GIEQSLTGLS ISSTPPAVSS VLSTGVPTVP LLPPQVNQSL TSVPPMNPAT TLPGLMPLPA GLPNLPNLNL NLPAPHIMPG VGLPELVNPG LPPLPSMPPR NLPGIAPLPL PSEFLPSFPL VPESSSAASS GELLSSLPPT SNAPSDPATT TAKADAASSL TVDVTPPTAK APTTVEDRVG DSTPVSEKPV SAAVDANASE SP (152-452 aa encoded by BC007770)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat testis tissue were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of GORASP2 antibody (10598-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GORASP2 transfected HeLa cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using GORASP2 antibody 10598-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (Product # 10598-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-Phalloidin (orange).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 10598-1-AP (GORASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 10598-1-AP (GORASP2 antibody) at dilution of 1:100 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-GORASP2 (IP:10598-1-AP, 3ug; Detection:10598-1-AP 1:700) with HeLa cells lysate 1320ug.