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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00026191-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00026191-M01, RRID:AB_830048
- Product name
- Mouse Monoclonal Lyp/PTPN22 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PTPN22 (4F6)
- Reactivity
- Human, Mouse, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Experimental ischemia/reperfusion model impairs endocannabinoid signaling and Na+/K+ ATPase expression and activity in kidney proximal tubule cells.
A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models.
Sampaio LS, Iannotti FA, Veneziani L, Borelli-Tôrres RT, De Maio F, Piscitelli F, Reis RAM, Di Marzo V, Einicker-Lamas M
Biochemical pharmacology 2018 Aug;154:482-491
Biochemical pharmacology 2018 Aug;154:482-491
A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models.
Dai X, James RG, Habib T, Singh S, Jackson S, Khim S, Moon RT, Liggitt D, Wolf-Yadlin A, Buckner JH, Rawlings DJ
The Journal of clinical investigation 2013 May;123(5):2024-36
The Journal of clinical investigation 2013 May;123(5):2024-36
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Analysis of PTPN22 over-expressed 293 cell line, cotransfected with PTPN22 Validated Chimera RNAi ( Cat # H00026191-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PTPN22 monoclonal antibody (M01), clone 4F6 (Cat # H00026191-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Analysis of PTPN22 expression in transfected 293T cell line by PTPN22 monoclonal antibody (M01), clone 4F6.Lane 1: PTPN22 transfected lysate(85.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Lyp/PTPN22 Antibody (4F6) [H00026191-M01] - Detection limit for recombinant GST tagged PTPN22 is 1 ng/ml as a capture antibody.
