Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- 12219-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DYNC1I2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: HCQSAVMSA TFAKFHPNLV VGGTYSGQIV LWDNRSNKRT PVQRTPLSAA AHTHPVYCVN VVGTQNAHNL ISISTDGKIC SWSLDMLSHP QDSMELVHKQ SKAVAVTSMS FPVGDVNNFV VGSEEGSVYT ACRHGSKAGI SEMFEGHQGP ITGIHCHAAV GAVDFSHLFV TSSFDWTVKL WTTKNNKPLY SFEDNADYVY DVMWSPTHPA LFACVDGMGR LDLWNLNNDT EVPTASISVE GNPALNRVRW THSGREIAVG DSEGQIVIYD VGEQIAVPRN DEWARFGRTL AEINANRADA EEEAATRIPA (330-638 aa encoded by BC015038)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.18 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A375 cells using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with mouse testis tissue lysate 4000ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DYNC1I2 (IP:12219-1-AP, 4 ug; Detection:12219-1-AP 1:500) with HeLa cells lysate 2080 ug.