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- Product number
- AF1725 - Provider product page
- Provider
- R&D Systems
- Product name
- Anti-Human Fetuin B Antigen Affinity-purified Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Mouse myeloma cell line NS0-derived recombinant human Fetuin B, Met19-Pro382
- Description
- Antigen Affinity-purified
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
Q9UGM5- Isotype
- IgG
- Vial size
- 100 µg
Submitted references The liver of woodchucks chronically infected with the woodchuck hepatitis virus contains foci of virus core antigen-negative hepatocytes with both altered and normal morphology.
Xu C, Yamamoto T, Zhou T, Aldrich CE, Frank K, Cullen JM, Jilbert AR, Mason WS
Virology 2007 Mar 15;359(2):283-94
Virology 2007 Mar 15;359(2):283-94
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Fetuin B by Western Blot. Western blot shows human serum, human placenta tissue lysate, mouse serum, and rat placenta tissue lysate. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat Fetuin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1725) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fetuin B at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Fetuin B by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and rat placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Fetuin B at approximately 64 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human/Mouse/Rat Fetuin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1725) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
