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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MAB17251 - Provider product page
- Provider
- R&D Systems
- Product name
- Anti-Human Fetuin B Monoclonal Antibody (Clone 212607)
- Antibody type
- Monoclonal
- Antigen
- Mouse myeloma cell line NS0-derived recombinant human Fetuin B, Met19-Pro382
- Description
- Protein A or G purified from hybridoma culture supernatant
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Q9UGM5- Isotype
- IgG
- Vial size
- 500 µg
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Fetuin B by Western Blot. Western blot shows human serum. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fetuin B at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Fetuin B by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Fetuin B at approximately 62 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
