10414-1-AP

antibody from Invitrogen Antibodies

Targeting: BCAS2 DAM1, Snt309, SPF27

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10414-1-AP

Provider

Invitrogen Antibodies

Product name

BCAS2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAGTGLVAG EVVVDALPYF DQGYEAPGVR EAAAALVEEE TRRYRPTKNY LSYLTAPDYS AFETDIMRNE FERLAARQPI ELLSMKRYEL PAPSSGQKND ITAWQECVNN SMAQLEHQAV RIENLELMSQ HGCNAWKVYN ENLVHMIEHA QKELQKLRKH IQDLNWQRKN MQLTAGSKLR EMESNWVSLV SKNYEIERTI VQLENEIYQI KQQHGEANKE NIRQDF (1-225 aa encoded by B C005285)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.34 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COS-7 cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HESC cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 10414-1-AP (BCAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells using 10414-1-AP (BCAS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:100 (under 25x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10414-1-AP (BCAS2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10414-1-AP ( BCAS2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-BCAS2 (IP:10414-1-AP, 3ug; Detection:10414-1-AP 1:1000) with mouse brain tissue lysate 6500ug.