Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LF-MA0176 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Gelsolin Monoclonal Antibody (35B2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- A suggested positive control for this product is human plasma.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 35B2
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), PANC-1 (Lane 2), T-47D (Lane 3), and human plasma (5 µL) (Lane 4). The blot was probed with Mouse Anti-Gelsolin Monoclonal Antibody (Product # LF-MA0176, 1:250 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 83 kDa band corresponding to Gelsolin was observed across the cell lines and samples tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).