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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX101185 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101185, RRID:AB_1950463
- Product name
- Gelsolin antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Sex hormone-binding globulin (SHBG) is a potential early diagnostic biomarker for gastric cancer.
The putative involvement of actin-binding proteins and cytoskeleton proteins in pathological mechanisms of ketamine cystitis-Revealed by a prospective pilot study using proteomic approaches.
Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: a cell model of heart ischemia-reperfusion injury and treatment.
Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients.
A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.
Cheng CW, Chang CC, Patria YN, Chang RT, Liu YR, Li FA, Shih HM, Lin CY
Cancer medicine 2018 Jan;7(1):64-74
Cancer medicine 2018 Jan;7(1):64-74
The putative involvement of actin-binding proteins and cytoskeleton proteins in pathological mechanisms of ketamine cystitis-Revealed by a prospective pilot study using proteomic approaches.
Yang HH, Zhai WJ, Kuo HC
Proteomics. Clinical applications 2017 Mar;11(3-4)
Proteomics. Clinical applications 2017 Mar;11(3-4)
Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: a cell model of heart ischemia-reperfusion injury and treatment.
Law CH, Li JM, Chou HC, Chen YH, Chan HL
Toxicology 2013 Jan 7;303:54-71
Toxicology 2013 Jan 7;303:54-71
Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients.
Lu CH, Lin ST, Chou HC, Lee YR, Chan HL
Archives of biochemistry and biophysics 2013 Jan 15;529(2):146-56
Archives of biochemistry and biophysics 2013 Jan 15;529(2):146-56
A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.
Yun-Hong Y, Chih-Fan C, Chia-Wei C, Yen-Chung C
Molecular & cellular proteomics : MCP 2011 Oct;10(10):M110.007138
Molecular & cellular proteomics : MCP 2011 Oct;10(10):M110.007138
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7.5% SDS PAGE GTX101185 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A:NIH-3T37.5% SDS PAGE GTX101185 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Gelsolin antibody detects Gelsolin protein by western blot analysis. Human tissue extract (30 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX101185) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Gelsolin(GTX101185) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Gelsolin(GTX101185) antibody at 1:500 dilution.
