RQ7898

antibody from NSJ Bioreagents

Targeting: OPA1 FLJ12460, KIAA0567, MGM1, NPG, NTG

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7898

Provider

NSJ Bioreagents

Product name

Dynamin-like 120 kDa protein Antibody / OPA1

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Dynamin-like 120 kDa protein antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human Ramos, 2) human HeLa, 3) human PANC-1, 4) human MCF7, 5) rat brain, 6) mouse eye and 7) mouse brain tissue lysate with Dynamin-like 120 kDa protein antibody. Predicted molecular weight: 111-120 kDa with multiple smaller isoforms from 81-95 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with Dynamin-like 120 kDa protein antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human Daudi cells with Dynamin-like 120 kDa protein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Dynamin-like 120 kDa protein antibody.