- PA1-16991 - Invitrogen Antibodies OPA1 antibody

Submitted references Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia.

Tolomeo AM, Fabozzo A, Malvicini R, De Lazzari G, Bisaccia P, Gaburro G, Arcidiacono D, Notarangelo D, Caicci F, Zanella F, Marchesan M, Yannarelli G, Santovito G, Muraca M, Gerosa G
Antioxidants (Basel, Switzerland) 2022 Feb 28;11(3)

Tau Deletion Prevents Cognitive Impairment and Mitochondrial Dysfunction Age Associated by a Mechanism Dependent on Cyclophilin-D.

Jara C, Cerpa W, Tapia-Rojas C, Quintanilla RA
Frontiers in neuroscience 2020;14:586710

Intracardiac administration of ephrinA1-Fc preserves mitochondrial bioenergetics during acute ischemia/reperfusion injury.

Torres MJ, McLaughlin KL, Renegar RH, Valsaraj S, Whitehurst KS, Sharaf OM, Sharma UM, Horton JL, Sarathy B, Parks JC, Brault JJ, Fisher-Wellman KH, Neufer PD, Virag JAI
Life sciences 2019 Dec 15;239:117053

Genetic ablation of tau improves mitochondrial function and cognitive abilities in the hippocampus.

Jara C, Aránguiz A, Cerpa W, Tapia-Rojas C, Quintanilla RA
Redox biology 2018 Sep;18:279-294

Quantitative proteomic and functional analysis of liver mitochondria from high fat diet (HFD) diabetic mice.

Guo Y, Darshi M, Ma Y, Perkins GA, Shen Z, Haushalter KJ, Saito R, Chen A, Lee YS, Patel HH, Briggs SP, Ellisman MH, Olefsky JM, Taylor SS
Molecular & cellular proteomics : MCP 2013 Dec;12(12):3744-58

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of OPA1 in human HeLa cells, mouse MEF cells and rat brain. Samples were incubated in OPA1 polyclonal antibody (Product # PA1-16991) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody. Separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The protein ladder was detected with an anti-mouse HRP secondary antibody. Detection: chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of OPA1 in post-nuclear extracts of mouse embryonic fibroblasts. Sample was incubated in OPA1 polyclonal antibody (Product # PA1-16991).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of OPA1 in 0.5 mg/mL MEF lysate. Samples were incubated in OPA1 polyclonal (Product # PA1-16991). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of OPA1 in prostatic smooth muscle and glandular epithelium. Samples were incubated in OPA1 polyclonal antibody (Product # PA1-16991). Human Prostate 40X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 Effects of tau deletion on the levels of proteins involved in mitochondrial dynamics and structure. ( A ) Representative western blot of hippocampal lysates and densitometric analysis of proteins involved in mitochondrial fusion, such as Mnf1, Mnf2, and Opa1. ( B ) Representative western blot of hippocampal lysates and densitometric analysis of proteins involved in mitochondrial fission, including phospho-Drp1, total Drp1, and Fis1. ( C ) Western blot of hippocampal lysates and densitometric analysis of proteins that form the mitochondrial permeability transition pore (mPTP), such as Cyp-D, VDAC, and ANT. ( D-F ) Relative mRNA expression of Cyp-D, VDAC, and ANT in the hippocampus of WT and tau -/- animals. *p < 0.05, **p < 0.01; data are presented as the mean +- SE. n = 4 different animals for WT group and n = 5 different animals for tau -/- group. Fig. 2

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of OPA1 in heart tissue. Quantitative results ( A ) and representative Western blots ( B ) of OPA1 of different heart regions treated with different cardioplegia solutions. TOM40 was used as loading control. Values are shown as median +- range, n = 3. BL: baseline; CC: cardioplegia; CC-ADN: cardioplegia supplemented with adenosine; NtC + CC: 250 mL warm cardioplegia + 750 mL cold cardioplegia; NtC-ADN + CC: 250 mL warm cardioplegia supplemented with adenosine + 750 mL cold cardioplegia.

PA1-16991

Antibody data