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- References [1]
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- Western blot [2]
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- Product number
- NBP1-06096 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-06096, RRID:AB_1556210
- Product name
- Goat Polyclonal B7-H4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references B7-H4 expression in renal cell carcinoma and tumor vasculature: associations with cancer progression and survival.
Krambeck AE, Thompson RH, Dong H, Lohse CM, Park ES, Kuntz SM, Leibovich BC, Blute ML, Cheville JC, Kwon ED
Proceedings of the National Academy of Sciences of the United States of America 2006 Jul 5;103(27):10391-10396
Proceedings of the National Academy of Sciences of the United States of America 2006 Jul 5;103(27):10391-10396
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B7-H4 Antibody [NBP1-06096] - (0.5ug/ml) staining of Human Pancrease lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B7-H4 Antibody [NBP1-06096] - Staining of HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B). Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D) with antibody at 1 ug/mL and Human pancreas iso 1 (E) lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B7-H4 Antibody [NBP1-06096] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
