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LearnA700-043
antibody from Invitrogen Antibodies
Targeting: VTCN1
B7-H4, B7H4, B7S1, B7x, FLJ22418
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- A700-043 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- B7-H4 Recombinant Rabbit Monoclonal Antibody (BLR043F)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR043F
- Vial size
- 100 µL
- Concentration
- 50 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human B7-H4 by western blot. Samples: Whole cell lysate (50 µg) from OVCAR-4, Hep-G2, MCF-7, HeLa, HEK293T, Jurkat, MOLT-4, HH, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody [BLR043F] (Product # A700-043 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human B7-H4 by immunocytochemistry.Sample: FFPE section of OVCAR-3 cells.Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody [BLR043F] (Product # A700-043 lot 1) used at 1:100.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human B7-H4 by immunohistochemistry. AntibodySample: FFPE section of ovarian carcinoma.Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (BLR043F) (A700-043 lot 1) used at 1:100.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human B7-H4 (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody [BLR043F] (Product # A700-043 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human B7-H4 (shaded) in human peripheral blood mononuclear cells by flow cytometry. Antibody: Rabbit anti-B7-H4 recombinant monoclonal [BLR043F] (Product #A700-043; lot 1) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human B7-H4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-4 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-B7-H4 recombinant monoclonal antibody [BLR043F] (Product # A700-043 lot 1) used for IP at 20 µL per reaction. B7-H4 was also immunoprecipitated by rabbit anti-B7-H4 recombinant monoclonal antibody [209-2B5]. For blotting immunoprecipitated B7-H4 (Product # A700-043) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.
