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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS3494 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS3494, RRID:AB_1661921
- Product name
- Ob (K36) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids N-terminus of Human Ob.
- Description
- Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of Ob (K36) pAb at 1:500 dilutionLane1:HEK293A whole cell lysateLane2:Raw264.7 whole cell lysateLane3:H9C2 whole cell lysate
