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- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF498 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Leptin/OB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Leptin/OB in ELISAs and Western blots. In sandwich ELISAs, approximately 40% cross-reactivity with recombinant rat Leptin/OB is observed and less than 0.2% cross-reactivity with recombinant human Leptin/OB is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC.
Elevated leptin disrupts epithelial polarity and promotes premalignant alterations in the mammary gland.
Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity.
Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain.
Leptin sustains spontaneous remyelination in the adult central nervous system.
Data on the expression of leptin and leptin receptor in the dorsal root ganglion and spinal cord after preganglionic cervical root avulsion.
Rapid linkage of innate immunological signals to adaptive immunity by the brain-fat axis.
Hypothalamic tanycytes are an ERK-gated conduit for leptin into the brain.
Leptin-dependent and leptin-independent paracrine effects of perivascular adipose tissue on neointima formation.
Leptin derived from adipocytes in injured peripheral nerves facilitates development of neuropathic pain via macrophage stimulation.
Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
John B, Naczki C, Patel C, Ghoneum A, Qasem S, Salih Z, Said N
Oncogene 2019 May;38(22):4366-4383
Oncogene 2019 May;38(22):4366-4383
Elevated leptin disrupts epithelial polarity and promotes premalignant alterations in the mammary gland.
Tenvooren I, Jenks MZ, Rashid H, Cook KL, Muhlemann JK, Sistrunk C, Holmes J, Wang K, Bonin K, Hodges K, Lo HW, Shaikh A, Camarillo IG, Lelièvre SA, Seewaldt V, Vidi PA
Oncogene 2019 May;38(20):3855-3870
Oncogene 2019 May;38(20):3855-3870
Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity.
Zeng Q, Luo X, Han M, Liu W, Li H
EBioMedicine 2018 Jun;32:43-49
EBioMedicine 2018 Jun;32:43-49
Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain.
Nozaki C, Nent E, Bilkei-Gorzo A, Zimmer A
Scientific reports 2018 Jul 17;8(1):10827
Scientific reports 2018 Jul 17;8(1):10827
Leptin sustains spontaneous remyelination in the adult central nervous system.
Matoba K, Muramatsu R, Yamashita T
Scientific reports 2017 Jan 16;7:40397
Scientific reports 2017 Jan 16;7:40397
Data on the expression of leptin and leptin receptor in the dorsal root ganglion and spinal cord after preganglionic cervical root avulsion.
Chang KT, Lin YL, Lin CT, Tsai MJ, Huang WC, Shih YH, Lee YY, Cheng H, Huang MC
Data in brief 2017 Dec;15:567-572
Data in brief 2017 Dec;15:567-572
Rapid linkage of innate immunological signals to adaptive immunity by the brain-fat axis.
Kim MS, Yan J, Wu W, Zhang G, Zhang Y, Cai D
Nature immunology 2015 May;16(5):525-33
Nature immunology 2015 May;16(5):525-33
Hypothalamic tanycytes are an ERK-gated conduit for leptin into the brain.
Balland E, Dam J, Langlet F, Caron E, Steculorum S, Messina A, Rasika S, Falluel-Morel A, Anouar Y, Dehouck B, Trinquet E, Jockers R, Bouret SG, Prévot V
Cell metabolism 2014 Feb 4;19(2):293-301
Cell metabolism 2014 Feb 4;19(2):293-301
Leptin-dependent and leptin-independent paracrine effects of perivascular adipose tissue on neointima formation.
Schroeter MR, Eschholz N, Herzberg S, Jerchel I, Leifheit-Nestler M, Czepluch FS, Chalikias G, Konstantinides S, Schäfer K
Arteriosclerosis, thrombosis, and vascular biology 2013 May;33(5):980-7
Arteriosclerosis, thrombosis, and vascular biology 2013 May;33(5):980-7
Leptin derived from adipocytes in injured peripheral nerves facilitates development of neuropathic pain via macrophage stimulation.
Maeda T, Kiguchi N, Kobayashi Y, Ikuta T, Ozaki M, Kishioka S
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 4;106(31):13076-81
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 4;106(31):13076-81
Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
European cytokine network 2002 Apr-Jun;13(2):225-9
European cytokine network 2002 Apr-Jun;13(2):225-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Leptin/OB in Mouse Brain. Leptin/OB was detected in perfusion fixed frozen sections of mouse brain using Mouse Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF498) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
