AF398

antibody from R&D Systems

Targeting: LEP OB, OBS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF398

Provider

R&D Systems

Product name

Human Leptin/OB Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Leptin/OB in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant mouse Leptin/OB is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q6NT58


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

UV-induced inhibition of adipokine production in subcutaneous fat aggravates dermal matrix degradation in human skin.

Kim EJ, Kim YK, Kim MK, Kim S, Kim JY, Lee DH, Chung JH
Scientific reports 2016 May 10;6:25616

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Increased leptin expression in endometriosis cells is associated with endometrial stromal cell proliferation and leptin gene up-regulation.

Wu MH, Chuang PC, Chen HM, Lin CC, Tsai SJ
Molecular human reproduction 2002 May;8(5):456-64

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Increased leptin expression in endometriosis cells is associated with endometrial stromal cell proliferation and leptin gene up-regulation.

Wu MH, Chuang PC, Chen HM, Lin CC, Tsai SJ
Molecular human reproduction 2002 May;8(5):456-64

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Recombinant Human, Mouse, and Rat Leptin/OB by Western Blot. Western blot shows 100 ng of Recombinant Human Leptin/OB (Catalog # 398-LP), Recombinant Mouse Leptin/OB (Catalog # 498-OB) and Recombinant Rat Leptin/OB (Catalog # 598-LP). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin/OB at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Leptin/OB in Human Stomach. Leptin/OB was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by Leptin/OB and Neutralization by Human Leptin/OB Antibody. Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with human Leptin R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Leptin/OB (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398). The ND₅₀ is typically 0.5-3.0 µg/mL.