PA5-142544

antibody from Invitrogen Antibodies

Targeting: HOXD13 HOX4I, SPD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-142544

Provider

Invitrogen Antibodies

Product name

HOXD13 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.

Reactivity

Human, Rat

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HOXD13 in Rat Brain lysate (35 µg protein in RIPA buffer) using HOXD13 Polyclonal Antibody (Product # PA5-142544) at 1 µg/mL. Detected by chemiluminescence.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HOXD13 in HEK293 cells using HOXD13 Polyclonal Antibody (Product # PA5-142544). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HOXD13 in U2OS cells using HOXD13 Polyclonal Antibody (Product # PA5-142544). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of HOXD13 in paraffin embedded Human Prostate using HOXD13 Polyclonal Antibody (Product # PA5-142544) at 8 µg/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.