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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00003284-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003284-M02, RRID:AB_1237061
- Product name
- Mouse Monoclonal Beta Hydroxysteroid Dehydrogenase Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. HSD3B2 - hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (1E8)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Pomegranate extracts impact the androgen biosynthesis pathways in prostate cancer models in vitro and in vivo.
Ming DS, Pham S, Deb S, Chin MY, Kharmate G, Adomat H, Beheshti EH, Locke J, Guns ET
The Journal of steroid biochemistry and molecular biology 2014 Sep;143:19-28
The Journal of steroid biochemistry and molecular biology 2014 Sep;143:19-28
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody (1E8) [H00003284-M02] - Analysis of HSD3B2 expression in transfected 293T cell line by HSD3B2 monoclonal antibody (M02), clone 1E8.Lane 1: HSD3B2 transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Beta Hydroxysteroid Dehydrogenase Antibody (1E8) [H00003284-M02] - Detection limit for recombinant GST tagged HSD3B2 is 0.3 ng/ml as a capture antibody.
