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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27791 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSD3B2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, Raji.
- Concentration
- 1.24 mg/mL
Submitted references Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line.
Torres MJ, López-Moncada F, Herrera D, Indo S, Lefian A, Llanos P, Tapia J, Castellón EA, Contreras HR
Oncology reports 2021 Aug;46(2)
Oncology reports 2021 Aug;46(2)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSD3B2 using A) 30 µg HeLa whole cell lysate and B) 30 µg Raji whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a HSD3B2 polyclonal antibody (Product # PA5-27791) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HSD3B2 Polyclonal Antibody (Product # PA5-27791). Mouse tissue extract (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HSD3B2 Polyclonal Antibody (Product # PA5-27791) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HSD3B2 in methanol-fixed HeLa cells using a HSD3B2 polyclonal antibody (Product # PA5-27791) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HSD3B2 Polyclonal Antibody detects HSD3B2 protein at cytoplasm on mouse ovart by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovart. HSD3B2 Polyclonal Antibody (Product # PA5-27791) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft , using HSD3B2 (Product # PA5-27791) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
