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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- H00003283-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003283-M01, RRID:AB_425493
- Product name
- HSD3B1 monoclonal antibody (M01), clone 3C11-D4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant HSD3B1.
- Antigen sequence
MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVL
DKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLK
RACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGT
QLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQN
GHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLK
NGGTLYTCALRPMYIYGEGSRFLSASINEALNNNG
ILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKK
APSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRL
DSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPF
NRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAK
QKTVEWVGSLVDRHKENLKSKTQ- Isotype
- IgG
- Antibody clone number
- 3C11-D4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Steroidogenic enzymes, their related transcription factors and nuclear receptors in human sebaceous glands under normal and pathological conditions.
Chorangiocarcinoma: a case report and review of the literature.
HSD3B1 as a novel trophoblast-associated marker that assists in the differential diagnosis of trophoblastic tumors and tumorlike lesions.
Trophogram, an immunohistochemistry-based algorithmic approach, in the differential diagnosis of trophoblastic tumors and tumorlike lesions.
Azmahani A, Nakamura Y, Felizola SJ, Ozawa Y, Ise K, Inoue T, McNamara KM, Doi M, Okamura H, Zouboulis CC, Aiba S, Sasano H
The Journal of steroid biochemistry and molecular biology 2014 Oct;144 Pt B:268-79
The Journal of steroid biochemistry and molecular biology 2014 Oct;144 Pt B:268-79
Chorangiocarcinoma: a case report and review of the literature.
Ariel I, Boldes R, Weintraub A, Reinus C, Beller U, Arbel R
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2009 May;28(3):267-71
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2009 May;28(3):267-71
HSD3B1 as a novel trophoblast-associated marker that assists in the differential diagnosis of trophoblastic tumors and tumorlike lesions.
Mao TL, Kurman RJ, Jeng YM, Huang W, Shih IeM
The American journal of surgical pathology 2008 Feb;32(2):236-42
The American journal of surgical pathology 2008 Feb;32(2):236-42
Trophogram, an immunohistochemistry-based algorithmic approach, in the differential diagnosis of trophoblastic tumors and tumorlike lesions.
Shih IeM
Annals of diagnostic pathology 2007 Jun;11(3):228-34
Annals of diagnostic pathology 2007 Jun;11(3):228-34
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HSD3B1 monoclonal antibody (M01), clone 3C11-D4. Western Blot analysis of HSD3B1 expression in human placenta.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HSD3B1 expression in transfected 293T cell line by HSD3B1 monoclonal antibody (M01), clone 3C11-D4.Lane 1: HSD3B1 transfected lysate (Predicted MW: 42.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to HSD3B1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of various tissues with hematoxylin and HSD3B1 antibody under high magnification.[antibody concentration 3 ug/ml]( a ) Stomach( b ) Esophagus( c ) Endometrium( d ) Uterine cervix( e ) Placenta( f ) Ovary, clear cell carcinoma( g ) Hepatocellular carcinoma( h ) Breast cancer( i ) Colon adenocarcinoma ( j and k ) Cervical carcinoma( l, m, and n ) Choriocarcinoma( o ) Epithelioid trophoblastic tumor.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
