11610-1-AP

antibody from Invitrogen Antibodies

Targeting: MTX2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11610-1-AP

Provider

Invitrogen Antibodies

Product name

MTX2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MYIAAEPWP ENATLYQQLK GEQILLSDNA ASLAVQAFLQ MCNLPIKVVC RANAEYMSPS GKVPFIHVGN QVVSELGPIV QFVKAKGHSL SDGLEEVQKA EMKAYMELVN NMLLTAELYL QWCDEATVGE ITHARYGSPY PWPLNHILAY QKQWEVKRKM KAIGWGKKTL DQVLEDVDQC CQALSQRLGT QPYFFNKQPT ELDALVFGHL YTILTTQLTN DELSEKVKNY SNLLAFCRRI EQHYFEDRGK GRLS (1-253 aa encoded by BC017271)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP (MTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat heart tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP (MTX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP ( MTX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP (MTX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using MTX2 antibody 11610-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using MTX2 antibody 11610-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11610-1-AP ( MTX2 antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11610-1-AP ( MTX2 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MTX2 (IP:11610-1-AP, 3ug; Detection:11610-1-AP 1:300) with mouse heart tissue lysate 4000ug.