Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX111643 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111643, RRID:AB_1950012
- Product name
- Collagen III antibody [C2C3-2], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references PDGFR Signaling Mediates Hyperproliferation and Fibrotic Responses of Subsynovial Connective Tissue Cells in Idiopathic Carpal Tunnel Syndrome.
Thy-1 dependent uptake of mesenchymal stem cell-derived extracellular vesicles blocks myofibroblastic differentiation.
Lyso-globotriaosylceramide downregulates KCa3.1 channel expression to inhibit collagen synthesis in fibroblasts.
Saito Y, Chikenji T, Ozasa Y, Fujimiya M, Yamashita T, Gingery A, Iba K
Scientific reports 2017 Nov 23;7(1):16192
Scientific reports 2017 Nov 23;7(1):16192
Thy-1 dependent uptake of mesenchymal stem cell-derived extracellular vesicles blocks myofibroblastic differentiation.
Shentu TP, Huang TS, Cernelc-Kohan M, Chan J, Wong SS, Espinoza CR, Tan C, Gramaglia I, van der Heyde H, Chien S, Hagood JS
Scientific reports 2017 Dec 22;7(1):18052
Scientific reports 2017 Dec 22;7(1):18052
Lyso-globotriaosylceramide downregulates KCa3.1 channel expression to inhibit collagen synthesis in fibroblasts.
Choi JY, Shin MY, Suh SH, Park S
Biochemical and biophysical research communications 2015 Dec 25;468(4):883-8
Biochemical and biophysical research communications 2015 Dec 25;468(4):883-8
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa 5% SDS PAGE GTX111643 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III alpha1 antibody [C2C3-2], C-term (GTX111643) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with COL3A1 antibody [C2C3-2], C-term (GTX111643) diluted at 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with COL3A1 antibody [C2C3-2], C-term (GTX111643) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Collagen III(GTX111643) antibody at 1:50 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Collagen III (GTX111643) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Collagen III antibody [C2C3-2], C-term detects Collagen III protein at secreted on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Collagen III antibody [C2C3-2], C-term (GTX111643) dilution: 1:500.