M00788

antibody from Boster Biological Technology

Targeting: COL3A1 EDS4A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

M00788

Provider

Boster Biological Technology

Product name

Anti-Collagen III/COL3A1 Antibody Picoband™ (monoclonal, 9H9)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for Collagen III/COL3A1 detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

9H9

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

References

Submitted references

A heparin-functionalized bioink with sustained delivery of vascular endothelial growth factor for 3D bioprinting of prevascularized dermal constructs.

Li M, Liu Z, Shen Z, Han L, Wang J, Sang S
International journal of biological macromolecules 2024 Mar;262(Pt 1):130075

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Involvement of oxidative stress-AMPK-Cx43-NLRP3 pathway in extracellular matrix remodeling of gastric smooth muscle cells in rats with diabetic gastroparesis.

Song B, Zhang G, Bao Y, Zhang M
Cell stress & chaperones 2024 Jun;29(3):440-455

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Impaired pulp healing associated with underlying disorders in the dental pulp of rats with type 2 diabetes.

Tao S, Yang T, Zhou JN, Zhang Q
Journal of dental sciences 2024 Jan;19(1):310-320

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Scutellarin ameliorates diabetic nephropathy via TGF-β1 signaling pathway.

Huang B, Han R, Tan H, Zhu W, Li Y, Jiang F, Xie C, Ren Z, Shi R
Natural products and bioprospecting 2024 Apr 24;14(1):25

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Scutellarin ameliorates diabetic nephropathy via TGF-β1 signaling pathway.

Huang B, Han R, Tan H, Zhu W, Li Y, Jiang F, Xie C, Shi R
Biological & pharmaceutical bulletin 2023 Oct 17;

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Epigallocatechin-3-Gallate Inhibits Atrial Fibrosis and Reduces the Occurrence and Maintenance of Atrial Fibrillation and its Possible Mechanisms.

Li T, Tong Q, Wang Z, Yang Z, Sun Y, Cai J, Xu Q, Lu Y, Liu X, Lin K, Qian Y
Cardiovascular drugs and therapy 2023 Mar 31;

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Radiation‑induced dysfunction of energy metabolism in the heart results in the fibrosis of cardiac tissues.

Xu P, Yi Y, Luo Y, Liu Z, Xu Y, Cai J, Zeng Z, Liu A
Molecular medicine reports 2021 Dec;24(6)

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ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes.

Fang PP, Pan CW, Lin W, Li J, Huang SS, Zhou GY, Du WJ, Li Q
Mediators of inflammation 2020;2020:8183713

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YKL-40 is highly expressed in the epicardial adipose tissue of patients with atrial fibrillation and associated with atrial fibrosis.

Wang Q, Shen H, Min J, Gao Y, Liu K, Xi W, Yang J, Yin L, Xu J, Xiao J, Wang Z
Journal of translational medicine 2018 Aug 15;16(1):229

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Mechanisms of nerve capping technique in prevention of painful neuroma formation.

Yan H, Zhang F, Kolkin J, Wang C, Xia Z, Fan C
PloS one 2014;9(4):e93973

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Rapamycin slows IgA nephropathy progression in the rat.

Tian J, Wang Y, Zhou X, Li Y, Wang C, Li J, Li R
American journal of nephrology 2014;39(3):218-29

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β-catenin is overexpressed in hepatic fibrosis and blockage of Wnt/β-catenin signaling inhibits hepatic stellate cell activation.

Ge WS, Wang YJ, Wu JX, Fan JG, Chen YW, Zhu L
Molecular medicine reports 2014 Jun;9(6):2145-51

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Collagen III/COL3A1 using anti- Collagen III/COL3A1 antibody (M00788). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: Hela whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Collagen III/COL3A1 antigen affinity purified monoclonal antibody (Catalog # M00788) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Collagen III/COL3A1 at approximately 180-190KD. The expected band size for Collagen III/COL3A1 is at 180-190KD.

Additional image