STJ23105

antibody from St John's Laboratory

Targeting: CFH ARMD4, ARMS1, FHL1, HF, HF1, HF2, HUS

Immunocytochemistry

Antibody data

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Validation data

Product number: STJ23105 - Provider product page
Provider: St John's Laboratory
Product name: Anti-CFH antibody (20-270) (STJ23105)
Antibody type: Polyclonal
Description: Rabbit polyclonal antibody anti-CFH (20-270) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: NA
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Handling: NA

CFH protein structure - STJ23105 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of extracts of various cell lines, using Complement factor H antibody (STJ23105) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of extracts of Rat liver, using Complement factor H antibody (STJ23105) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of extracts of Human plasma, using Complement factor H antibody (STJ23105) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of HepG2 cells using Complement factor H rabbit polyclonal antibody (STJ23105) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human liver cancer using Complement factor H rabbit polyclonal antibody (STJ23105) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human placenta using Complement factor H rabbit polyclonal antibody (STJ23105) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA